Journal article
Real-time tracking of cell cycle progression during CD8 effector and memory T-cell differentiation
I Kinjyo, J Qin, SY Tan, CJ Wellard, P Mrass, W Ritchie, A Doi, LL Cavanagh, M Tomura, A Sakaue-Sawano, O Kanagawa, A Miyawaki, PD Hodgkin, W Weninger
Nature Communications | NATURE PORTFOLIO | Published : 2015
DOI: 10.1038/ncomms7301
Open access
Abstract
The precise pathways of memory T-cell differentiation are incompletely understood. Here we exploit transgenic mice expressing fluorescent cell cycle indicators to longitudinally track the division dynamics of individual CD8+ T cells. During influenza virus infection in vivo, naive T cells enter a CD62L intermediate state of fast proliferation, which continues for at least nine generations. At the peak of the anti-viral immune response, a subpopulation of these cells markedly reduces their cycling speed and acquires a CD62L hi central memory cell phenotype. Construction of T-cell family division trees in vitro reveals two patterns of proliferation dynamics. While cells initially divide rapidl..
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Awarded by National Institutes of Health
Funding Acknowledgements
We thank members of the Weninger and Hodgkin laboratories for discussions, the Centenary Institute Flow Cytometry/Imaging Facility for cell sorting and microscopy, Animal Facility for mouse housing, Dr S. Turner (University of Melbourne) for providing PR8-OVA influenza virus, Dr D. Day (Swinburne University of Technology) and Microsurfaces Pty. Ltd. for providing the microgrids and the Ramaciotti Center at UNSW for microarray analysis. We acknowledge Ms R. Barugahare, M. Rizk and A. Cooray for maintaining mouse strains. This work was supported by a grant from the National Health and Medical Research Council (APP1030145 to W.W.) and a contract from the National Institutes of Health (BAA-NIAID-DAIT-HHSN272201100018C to W.W.).