Journal article

Separation of events mediating B cell proliferation and Ig production by using T cell membranes and lymphokines.

PD Hodgkin, LC Yamashita, RL Coffman, MR Kehry

The Journal of Immunology | Published : 1990


The initiation by Th cells of B cell proliferation and differentiation to produce Ig involves both cell contact- and lymphokine-mediated signals. Plasma membrane-enriched fractions from stimulated, but not unstimulated, Th cells induced Ag nonspecific and MHC unrestricted proliferation of 60 to 70% of small dense B cells. Induction of stimulatory membrane activity was inhibited by cycloheximide, and the activity was eliminated by both protease and heat treatment of membranes. Membrane-stimulated B cells did not differentiate to secrete Ig; however, addition of a lymphokine-containing supernatant from activated Th cells or the combination of IL-4 and IL-5 resulted in substantial Ig production..

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University of Melbourne Researchers