Switch recombination and germ-line transcription are division-regulated events in B lymphocytes.
MN McCall, PD Hodgkin
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism | Published : 1999
Treatment of resting murine B lymphocytes with CD40 ligand (CD40L) and IL-4 induces proliferation and a switch in immunoglobulin (Ig) isotype surface expression from IgM and IgD to IgG1 and IgE. Using a fluorescent dye to enable cell sorting according to cell division cycle number, we have examined molecular events associated with B cell differentiation, namely, germ-line transcription and DNA recombination. Digestion-circularisation polymerase chain reaction experiments showed that DNA recombination leading to isotype switching from IgM to IgG1 surface expression is division-dependent and was first detected after B cells had divided three times. Similarly, DNA rearrangement involving the Ig..View full abstract