Journal article

Cytokine regulation of glutamate decarboxylase biosynthesis in isolated rat islets of Langerhans

RS Schmidli, BE Faulkner-Jones, LC Harrison, RFL James, HJ DeAizpurua

Biochemical Journal | Published : 1996


Insulin-dependent diabetes mellitus (IDDM) is an autoimmune disease in which cytokines are thought to play an important role in β-cell destruction and immune regulation. A major target of β-cell autoimmunity in IDDM is the enzyme glutamate decarboxylase (GAD). We hypothesized that cytokines in the insulitis lesion modulate the synthesis of GAD. This may, in turn, modify the rate of β-cell destruction. Accordingly we cultured rat islets in the presence and absence of cytokines, and measured synthesis of both isoforms of GAD, GAD65 and GAD67, by [35S]methionine incorporation and immunoprecipitation with a rabbit antiserum that recognizes both GAD65 and GAD67. Incubation of islets with interleu..

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University of Melbourne Researchers