Journal article

Molecular cloning of full-length glutamic acid decarboxylase 67 from human pancreas and islets.

K Yamashita, DS Cram, LC Harrison

Biochem Biophys Res Commun | Published : 1993

Abstract

Glutamic acid decarboxylase (GAD) is a pancreatic islet autoantigen in insulin-dependent diabetes (IDD). Two forms of GAD, GAD65 and GAD67, have been identified in brain but human islets have been reported to express only GAD65. We have isolated full length GAD67 cDNA by polymerase chain reaction (PCR) cloning from human pancreas. Sequence analysis reveals only four nucleotide differences between human pancreas and brain GAD67, two of which result in amino acid changes. These differences are probably individual-specific and reflect allelic variation. Using mRNA from isolated human islets a partial sequence was obtained by PCR cloning that was identical to the midregion of pancreas GAD67 cDNA..

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University of Melbourne Researchers