Report from the 1st International NOD Mouse T-Cell Workshop and the follow-up mini-workshop.
DL Kaufman, R Tisch, N Sarvetnick, L Chatenoud, LC Harrison, K Haskins, A Quinn, E Sercarz, B Singh, M von Herrath, D Wegmann, L Wen, D Zekzer
Diabetes | Published : 2001
A workshop on autoreactive T-cell responses in NOD mice was held to optimize autoreactive T-cell detection methodologies. Using different proliferation assay protocols, 1 of the 11 participating laboratories detected spontaneous T-cell responses to GAD(524-543) and insulin(9-23) in their NOD mice. Two other laboratories were able to detect autoreactive responses when using enzyme-linked immunospot assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA) analysis of cytokines in culture supernatants, suggesting that these assays provided greater sensitivity. To address the divergent findings, a follow-up mini-workshop tested NOD mice from four different colonies side-by-side for T-cell p..View full abstract