Multiscale Structural Analysis of Plant ER-PM Contact Sites
Heather E McFarlane, Eun Kyoung Lee, Laura S Van Bezouwen, Bradford Ross, Abel Rosado, A Lacey Samuels
PLANT AND CELL PHYSIOLOGY | OXFORD UNIV PRESS | Published : 2017
Membrane contact sites are recognized across eukaryotic systems as important nanostructures. Endoplasmic reticulum (ER)-plasma membrane (PM) contact sites (EPCS) are involved in excitation-contraction coupling, signaling, and plant responses to stress. In this report, we perform a multiscale structural analysis of Arabidopsis EPCS that combines live cell imaging, quantitative transmission electron microscopy (TEM) and electron tomography over a developmental gradient. To place EPCS in the context of the entire cortical ER, we examined green fluorescent protein (GFP)-HDEL in living cells over a developmental gradient, then Synaptotagmin1 (SYT1)-GFP was used as a specific marker of EPCS. In al..View full abstract
Awarded by Natural Sciences and Engineering Research Council (NSERC)
This work was funded by the Natural Sciences and Engineering Research Council (NSERC) [Canadian Graduate Scholarship-D3 and an NSERC Post-Doctoral Fellowship (PDF-454454-2014) to H. E. M, and NSERC Discovery Grants to A.L.S. and A.R.].