Journal article

Multiscale Structural Analysis of Plant ER-PM Contact Sites

Heather E McFarlane, Eun Kyoung Lee, Laura S Van Bezouwen, Bradford Ross, Abel Rosado, A Lacey Samuels

PLANT AND CELL PHYSIOLOGY | OXFORD UNIV PRESS | Published : 2017

Abstract

Membrane contact sites are recognized across eukaryotic systems as important nanostructures. Endoplasmic reticulum (ER)-plasma membrane (PM) contact sites (EPCS) are involved in excitation-contraction coupling, signaling, and plant responses to stress. In this report, we perform a multiscale structural analysis of Arabidopsis EPCS that combines live cell imaging, quantitative transmission electron microscopy (TEM) and electron tomography over a developmental gradient. To place EPCS in the context of the entire cortical ER, we examined green fluorescent protein (GFP)-HDEL in living cells over a developmental gradient, then Synaptotagmin1 (SYT1)-GFP was used as a specific marker of EPCS. In al..

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University of Melbourne Researchers

Grants

Awarded by Natural Sciences and Engineering Research Council (NSERC)


Funding Acknowledgements

This work was funded by the Natural Sciences and Engineering Research Council (NSERC) [Canadian Graduate Scholarship-D3 and an NSERC Post-Doctoral Fellowship (PDF-454454-2014) to H. E. M, and NSERC Discovery Grants to A.L.S. and A.R.].