Journal article

Analyte stability during the total testing process: studies of vitamins A, D and E by LC-MS/MS

Ali A Albahrani, Victor Rotarou, Peter J Roche, Ronda F Greaves

CLINICAL CHEMISTRY AND LABORATORY MEDICINE | WALTER DE GRUYTER GMBH | Published : 2016

Abstract

BACKGROUND: There are limited evidence based studies demonstrating the stability of fat-soluble vitamins (FSV) measured in blood. This study aimed to examine the effects of light, temperature and time on vitamins A, D and E throughout the total testing process. METHODS: Four experiments were conducted. Three investigated the sample matrix, of whole blood, serum and the extracted sample, against the variables of temperature and light; and the fourth experiment investigated the sample during the extraction process against the variable of light. All samples were analysed via our simultaneous FSV method using liquid chromatography-tandem mass spectrometry technology. The allowable clinical perce..

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