Journal article

Anaerobic ammonium oxidation in sediments of surface flow constructed wetlands treating swine wastewater

Liang Chen, Feng Liu, Fen Jia, Ya-jun Hu, Cui Lai, Xi Li, Pei Luo, Run-lin Xiao, Yong Li, Jin-shui Wu

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | SPRINGER | Published : 2017

Abstract

Anaerobic ammonium oxidation (anammox) was suggested to be involved in the nitrogen (N) removal process in constructed wetlands (CWs). Nevertheless, its occurrence and role in CWs treating swine wastewater have not been well evaluated yet. In this study, we investigated the diversity, activity, and role of anammox bacteria in sediments of mesoscale surface flow CWs (SFCWs) subjected to different N loads of swine wastewater. We found that anammox bacteria were abundant in SFCW sediments, as indicated by 7.5 × 105 to 3.5 × 106 copies of the marker hzsB gene per gram of dry soil. Based on stable isotope tracing, potential anammox rates ranged from 1.03 to 12.5 nmol N g-1 dry soil h-1, accountin..

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Grants

Awarded by China Postdoctoral Science Foundation


Awarded by National Natural Science Foundation of China


Awarded by National Science and Technology Supporting Project


Awarded by CAS


Funding Acknowledgements

We sincerely thank Hu Li and San'an Nie (Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Science, Xiamen, China) for their skillful assistance of the <SUP>15</SUP>N isotope-pairing experiments. Special thanks to Public Service Technology Center, Institute of Subtropical Agriculture, Chinese Academy of Sciences, for instrument support. This study was funded by the China Postdoctoral Science Foundation Funded Project (2014M560648, 2015T80878), the National Natural Science Foundation of China (41601272), the National Science and Technology Supporting Project (2014BAD14B05), and the key CAS Programs (KZZD-EW-11).