Comparison and validation of ELISA assays for plasma insulin-like growth factor-1 in the horse
Courtnay L Baskerville, Nicholas J Bamford, Patricia A Harris, Simon R Bailey
OPEN VETERINARY JOURNAL | UNIV TRIPOLI, FAC VET MED | Published : 2017
Insulin-like growth factor-1 (IGF-1) plays several important physiological roles, and IGF-related pathways have been implicated in developmental osteochondral disease and endocrinopathic laminitis. This factor is also a downstream marker of growth hormone activity and its peptide mimetics. Unfortunately, previously used assays for measuring equine IGF-1 (radioimmunoassays and ELISAs) are no longer commercially available, and many of the kits on the market give poor results when used on horse samples. The aim of the present study was to compare three different ELISA assays (two human and one horse-specific). Plasma samples from six Standardbreds, six ponies and six Andalusians were used. The ..View full abstract
Related Projects (2)
Awarded by Australian Research Council
The authors would like to thank Miss Samantha Potter and Ms Lianne Salerno for their technical assistance and care of the animals in this project. This study was supported by the Australian Research Council (project number LP100200224) and The WALTHAM Centre for Pet Nutrition.