Journal article

Global analyses of mRNA translational control during early Drosophila embryogenesis

Xiaoli Qin, Soyeon Ahn, Terence P Speed, Gerald M Rubin

GENOME BIOLOGY | BMC | Published : 2007


BACKGROUND: In many animals, the first few hours of life proceed with little or no transcription, and developmental regulation at these early stages is dependent on maternal cytoplasm rather than the zygotic nucleus. Translational control is critical for early Drosophila embryogenesis and is exerted mainly at the gene level. To understand post-transcriptional regulation during Drosophila early embryonic development, we used sucrose polysomal gradient analyses and GeneChip analysis to illustrate the translation profile of individual mRNAs. RESULTS: We determined ribosomal density and ribosomal occupancy of over 10,000 transcripts during the first ten hours after egg laying. CONCLUSION: We rep..

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University of Melbourne Researchers