Journal article

A STUDY OF ISOLATION PROCEDURES FOR MULTIPLE INFECTIONS OF SALMONELLA AND ARIZONA IN A WILD MARSUPIAL, THE QUOKKA (SETONIX-BRACHYURUS)

RP HART, JB IVESON, SD BRADSHAW, TP SPEED

JOURNAL OF APPLIED BACTERIOLOGY | WILEY | Published : 1982

Abstract

Rectal swabs and faeces were used in the regular sampling for salmonellas and Arizonas from a heavily‐infected population of a marsupial, the quokka (Setonix brachyurus). The media used were strontium selenite A and strontium chloride B enrichment broths, with subculture onto modified bismuth sulphite agar and deoxycholate citrate agar. A study of sampling, enrichment, sub‐culture and colony selection procedures produced an optimal scheme giving high yields but consistent with reasonable economy of time and materials. A three‐swab sample was taken and inoculated into the two enrichment media, and with each enrichment subjected to three subcultures. The absolute efficiency of this procedure w..

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