Journal article

DEVELOPMENT OF INCREASED SERUM IMMUNOBLOT REACTIVITY AGAINST A 45,000-DALTON POLYPEPTIDE OF TREPONEMA-PALLIDUM (NICHOLS) CORRELATES WITH ESTABLISHMENT OF CHANCRE IMMUNITY IN SYPHILITIC RABBITS

RA STRUGNELL, WF WILLIAMS, L DRUMMOND, JS PEDERSEN, BH TOH, S FAINE

Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 1986

Abstract

Rabbits developed chancre immunity 5.0 to 7.5 weeks after intradermal infection with 10(3) Treponema pallidum (Nichols). The serological response against T. pallidum antigen during this 2.5-week period was examined by Western immunoblotting. Sera from rabbits infected for 5.0 weeks contained antibodies against 7 of 13 major T. pallidum immunogens, with strongest binding detected against a polypeptide of Mr 47,000. By 7.5 weeks of infection, syphilitic rabbit sera recognized 10 of 13 antigens; the most evident increase in serological reactivity was directed against a polypeptide of Mr 45,000, suggesting that the development of a strong serological response against this polypeptide correlated ..

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University of Melbourne Researchers