Induction of virulence factors in Giardia duodenalis independent of host attachment.
Samantha J Emery, Mehdi Mirzaei, Daniel Vuong, Dana Pascovici, Joel M Chick, Ernest Lacey, Paul A Haynes
Sci Rep | Published : 2016
Giardia duodenalis is responsible for the majority of parasitic gastroenteritis in humans worldwide. Host-parasite interaction models in vitro provide insights into disease and virulence and help us to understand pathogenesis. Using HT-29 intestinal epithelial cells (IEC) as a model we have demonstrated that initial sensitisation by host secretions reduces proclivity for trophozoite attachment, while inducing virulence factors. Host soluble factors triggered up-regulation of membrane and secreted proteins, including Tenascins, Cathepsin-B precursor, cystatin, and numerous Variant-specific Surface Proteins (VSPs). By comparison, host-cell attached trophozoites up-regulated intracellular pathw..View full abstract