Journal article

Differential regulation of protein tyrosine kinase signalling by Dock and the PTP61F variants

Lee F Willoughby, Jan Manent, Kirsten Allan, Han Lee, Marta Portela, Florian Wiede, Coral Warr, Tzu-Ching Meng, Tony Tiganis, Helena E Richardson

FEBS JOURNAL | WILEY | Published : 2017

Abstract

Tyrosine phosphorylation-dependent signalling is coordinated by the opposing actions of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs). There is a growing list of adaptor proteins that interact with PTPs and facilitate the dephosphorylation of substrates. The extent to which any given adaptor confers selectivity for any given substrate in vivo remains unclear. Here we have taken advantage of Drosophila melanogaster as a model organism to explore the influence of the SH3/SH2 adaptor protein Dock on the abilities of the membrane (PTP61Fm)- and nuclear (PTP61Fn)-targeted variants of PTP61F (the Drosophila othologue of the mammalian enzymes PTP1B and TCPTP respectively)..

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Grants

Awarded by Australian Research Council (ARC)


Awarded by ARC Discovery Project grant


Funding Acknowledgements

We thank Peter Burke for helping us to maintain the Drosophila stocks and all members of our laboratories for constructive discussions. We acknowledge the support of the Microscopy Core at the Peter MacCallum Cancer Centre and the Bioimaging facility at the La Trobe Institute of Molecular Science (LIMS), La Trobe University. HER was supported by a National Health and Medical Research Council (NHMRC) Senior Research Fellowship Level B and in institutional funds from LIMS and La Trobe University, TT by a NHMRC Principal Research Fellowship, MP by a Cancer Council Victoria grant to HER, LFW and KA by an Australian Research Council (ARC) Discovery Project grant (DPP120103943) to TT and HER, and JM by institutional funds from LIMS and La Trobe University and from an ARC Discovery Project grant (DP170102549) to HER and TT.