Journal article

Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species

Ali Kiani-Pouya, Ute Roessner, Nirupama S Jayasinghe, Adrian Lutz, Thusitha Rupasinghe, Nadia Bazihizina, Jennifer Bohm, Sulaiman Alharbi, Rainer Hedrich, Sergey Shabala



Epidermal bladder cells (EBCs) have been postulated to assist halophytes in coping with saline environments. However, little direct supporting evidence is available. Here, Chenopodium quinoa plants were grown under saline conditions for 5 weeks. One day prior to salinity treatment, EBCs from all leaves and petioles were gently removed by using a soft cosmetic brush and physiological, ionic and metabolic changes in brushed and non-brushed leaves were compared. Gentle removal of EBC neither initiated wound metabolism nor affected the physiology and biochemistry of control-grown plants but did have a pronounced effect on salt-grown plants, resulting in a salt-sensitive phenotype. Of 91 detected..

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Awarded by ARC Discovery

Awarded by Marie Curie Fellowship

Funding Acknowledgements

This work was supported by the ARC Discovery grant DP150101663 to S. Shabala and R. Hedrich and funding provided by King Saud University. N. Bazihizina is a recipient of the Marie Curie Fellowship (grant 700001). Metabolite analyses were conducted at Metabolomics Australia (School of BioSciences, The University of Melbourne, Australia), a NCRIS initiative under Bioplatforms Australia Pty Ltd. The authors thank Mrs Himasha Mendis of Metabolomics Australia for sample extraction, GC-MS untargeted data acquisition and analysis. We also thank Dr Tracey Cuin for her technical assistance in preparing this MS.