Journal article
3D Bioprinting Human Induced Pluripotent Stem Cell Constructs for In Situ Cell Proliferation and Successive Multilineage Differentiation
Q Gu, E Tomaskovic-Crook, GG Wallace, JM Crook
Advanced Healthcare Materials | Published : 2017
Abstract
The ability to create 3D tissues from induced pluripotent stem cells (iPSCs) is poised to revolutionize stem cell research and regenerative medicine, including individualized, patient-specific stem cell-based treatments. There are, however, few examples of tissue engineering using iPSCs. Their culture and differentiation is predominantly planar for monolayer cell support or induction of self-organizing embryoids (EBs) and organoids. Bioprinting iPSCs with advanced biomaterials promises to augment efforts to develop 3D tissues, ideally comprising direct-write printing of cells for encapsulation, proliferation, and differentiation. Here, such a method, employing a clinically amenable polysacch..
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Awarded by Australian National Fabrication Facility
Funding Acknowledgements
J.M.C., G.G.W., and Q.Z. conceived the study. J.M.C., Q.G., and E.T.-C. planned and/or executed experiments. J.M.C. wrote the paper with contributions from all authors. The authors wish to acknowledge funding from the Australian Research Council (ARC) Centre of Excellence Scheme (CE140100012), the use of facilities at the University of Wollongong Electron Microscopy Centre, support of the Australian National Fabrication Facility (ANFF)-Materials Node, and assistance provided by Situ Abdul Rahim with Flow Cytometry. Professor Gordon Wallace acknowledges the support of the ARC through an ARC Laureate Fellowship (FL110100196).