Journal article

Automated Cell Culture Systems and Their Applications to Human Pluripotent Stem Cell Studies

Maciej Daniszewski, Duncan E Crombie, Rachael Henderson, Helena H Liang, Raymond CB Wong, Alex W Hewitt, Alice Pebay



Pluripotent stem cells are an extremely powerful tool in modeling human diseases and hold much promise for personalized regenerative or cell replacement therapies. There is an increasing need for reproducible large-scale stem cell and differentiated progeny production, with minimal variation, rendering manual approaches impracticable. Here, we provide an overview of systems currently available for automated stem cell culture, and undertake a review of their capacities, capabilities, and relative limitations. With the merging of modern technology and stem cell biology, an increased demand and implementation of automated platforms for stem cell studies is anticipated.


Awarded by Australian Research Council (ARC) Future Fellowship

Funding Acknowledgements

The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by grants from the Ophthalmic Research Institute of Australia and Retina Australia. Further support was provided by an UROP-Stem Cells Australia scholarship (RH), an International Postgraduate Award Scholarship (MD), an NHMRC Fellowship (AWH), an Australian Research Council (ARC) Future Fellowship (AP, FT140100047), a MAWA fellowship (RCBW), and University of Melbourne and Operational Infrastructure Support from the Victorian government.