Journal article
Prognostic microRNAs in upper tract urothelial carcinoma: Multicenter and international validation study
L Izquierdo, R Montalbo, M Ingelmo-Torres, C Mallofré, M Ramírez-Backhaus, J Rubio, AG Van der Heijden, E Schaafsma, A Lopez-Beltran, A Blanca, N Lawrentschuk, A Alcaraz, L Mengual
Oncotarget | IMPACT JOURNALS LLC | Published : 2017
Abstract
Objective: To validate previously discovered miRNAs (miR-31-5p and miR-149- 5p) as prognostic factors for UTUC in an independent cohort of UTUC patients. Patients and Methods: Multicenter, international and retrospective study of formalinfixed paraffin-embedded tissue samples from 103 UTUC patients (45 progressing and 58 non-progressing) who underwent radical nephroureterectomy. Total RNA was isolated and reverse transcribed. The expression of target miRNAs (miR-31-5p and miR-149-5p) and the endogenous control miR-218-5p was evaluated in all samples by reverse transcription quantitative PCR. Normalized miRNA expression values were evaluated by multivariate forward stepwise Cox regression ana..
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Funding Acknowledgements
The present work was supported by a grant from the Spanish Urological Association (FIU 2010 to LI). Furthermore funding from CERCA Programme/Generalitat de Catalunya is gratefully acknowledged.