Journal article

Evaluating the Genomic Yield of a Single Endobronchial Ultrasound-guided Transbronchial Needle Aspiration in Lung Cancer: Meeting the Challenge of Doing More With Less

Tracy L Leong, Michael Christie, Sevastjan Kranz, Kym Pham, Arthur Hsu, Louis B Irving, Marie-Liesse Asselin-Labat, Daniel P Steinfort



BACKGROUND: Minimally invasive techniques, including endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA), yield small specimens that are adequate for cytologic diagnosis of lung cancer, but also need to provide material for molecular analysis to guide treatment. The number of EBUS-TBNA passes needed for mutation testing remains unclear. We sought to assess the adequacy of a single pass for genomic profiling of actionable mutations. METHODS: In a prospective observational study, paired samples from the same lesion were obtained from patients undergoing EBUS-TBNA for lung cancer diagnosis/staging. Following tumor cell confirmation by rapid on-site evaluation, a "refere..

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Funding Acknowledgements

The authors thank Aliaksei Holik and Stephen Wilcox from the Walter and Eliza Hall Institute of Medical Research for their technical expertise and advice in DNA extraction and assessment. The authors thank the Victorian Cancer Biobank and the Royal Melbourne Hospital Department of Anatomical Pathology for their technical assistance in specimen handling and processing. This work was supported by the Victorian State Government Operational Infrastructure Support, the Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme, and the Ian Potter Centre for Genomics and Personalised Medicine.