Journal article

A proteomic characterization shows differences in the milk fat globule membrane of buffalo and bovine milk

Hanh TH Nguyen, Lydia Ong, Ashfaqul Hoque, Sandra E Kentish, Nicholas Williamson, Ching-Seng Ang, Sally L Gras

Food Bioscience | Elsevier | Published : 2017

Abstract

The proteins of the milk fat globule membrane (MFGM) have a number of functions, such as the regulation of milk fat secretion and metabolism, the uptake and transportation of fatty acids in the intestine, and potential protection from bacterial or viral infection. While the proteome of the MFGM in bovine milk has been extensively characterized, knowledge of these proteins in buffalo milk is limited. In this study, a proteomic approach was used to characterize the proteome of the buffalo MFGM. Multiple extraction techniques were used to increase the number of proteins identified, while label free relative quantitative liquid chromatography tandem mass spectrometry was used for comparison betw..

View full abstract

Grants

Awarded by University of Melbourne


Awarded by University of Queensland


Awarded by Dairy Innovation Australia


Funding Acknowledgements

The authors acknowledge the Australian Government for providing an Australian Postgraduate Award (International) (APA - International) scholarship, the Rural Industries Research and Development Cooperation (RIRDC) for financial support, Paul O'Donnell for his assistance with experimental design and interpretation of mass spectrometry data and also Shaw River and La Latteria for supplying the buffalo and bovine milk used in this study. The authors also thank the Mass Spectrometry and Proteomics Facility of the Bio21 Molecular Science and Technology Institute and Associate Professor Heung-Chin Cheng's laboratory for access to equipment. Dr Hanh Nguyen, Dr Lydia Ong, Professor Sandra Kentish and Associate Professor Sally Gras are supported by the ARC Dairy Innovation Hub, a collaboration between the University of Melbourne, the University of Queensland and Dairy Innovation Australia [IH20100005].