The efficient synthesis and purification of amyloid-beta(1-42) using an oligoethylene glycol-containing photocleavable lysine tag
John A Karas, Asif Noor, Christine Schieber, Timothy U Connell, Frances Separovic, Paul S Donnelly
CHEMICAL COMMUNICATIONS | ROYAL SOC CHEMISTRY | Published : 2017
A short, monodisperse oligoethylene glycol-containing photocleavable lysine tag was developed to facilitate the efficient purification of hydrophobic and fibril-forming peptides. This new tag was used to prepare a modified Aβ42 peptide with increased solubility and decreased propensity to aggregate in aqueous media. The solubilising tag was readily removed by irradiation with UV light and permitted the preparation and isolation of Aβ42 in high purity and yield.
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We would like to acknowledge the Australian Research Council for financial support of this research.