Journal article
A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics
I Gallego Romero, BJ Pavlovic, I Hernando-Herraez, X Zhou, MC Ward, NE Banovich, CL Kagan, JE Burnett, CH Huang, A Mitrano, CI Chavarria, I Friedrich Ben-Nun, Y Li, K Sabatini, TR Leonardo, M Parast, T Marques-Bonet, LC Laurent, JF Loring, Y Gilad
eLife | Published : 2015
DOI: 10.7554/eLife.07103
Open access
Abstract
Comparative genomics studies in primates are restricted due to our limited access to samples. In order to gain better insight into the genetic processes that underlie variation in complex phenotypes in primates, we must have access to faithful model systems for a wide range of cell types. To facilitate this, we generated a panel of 7 fully characterized chimpanzee induced pluripotent stem cell (iPSC) lines derived from healthy donors. To demonstrate the utility of comparative iPSC panels, we collected RNA-sequencing and DNA methylation data from the chimpanzee iPSCs and the corresponding fibroblast lines, as well as from 7 human iPSCs and their source lines, which encompass multiple populati..
View full abstractGrants
Awarded by National Institutes of Health (NIH)
Awarded by California Institute for Regenerative Medicine (CIRM)
Awarded by European Research Council (ERC)
Awarded by Ministerio de Economia y Competitividad
Awarded by EMBO
Awarded by National Center for Advancing Translational Sciences
Funding Acknowledgements
Wellcome Trust Sir Henry Wellcome Postdoctoral Fellowship Irene Gallego RomeroNational Institutes of Health (NIH) GM077959 Yoav GiladCalifornia Institute for Regenerative Medicine (CIRM) CL1-00502 and TR01250 Jeanne F LoringEuropean Research Council (ERC) Starting Grant 260372 Tomas Marques-BonetMinisterio de Economia y Competitividad BFU2011-28549 Tomas Marques-BonetUniversity of California, San Diego, Department of Reproductive Medicine Louise C Laurent, Trevor R Leonardo, Karen SabatiniGeneralitat de Catalunya Irene Hernando-HerraezNational Institutes of Health (NIH) Training Grant GM007197 and F31 AG044948 Nicholas E BanovichNational Institutes of Health (NIH) K12 HD001259 Louise C LaurentEMBO ALTF 751-2014 Michelle C WardNational Center for Advancing Translational Sciences Clinical and Translational Science Awards, TL1 predoctoral fellowship TR 432-7 Courtney L Kagan