Overexpression of Human CD55 and CD59 or Treatment with Human CD55 Protects against Renal Ischemia-Reperfusion Injury in Mice
Anjan K Bongoni, Bo Lu, Evelyn J Salvaris, Veena Roberts, Doreen Fang, Jennifer L Mcrae, Nella Fisicaro, Karen M Dwyer, Peter J Cowan
JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2017
Deficiency in the membrane-bound complement regulators CD55 and CD59 exacerbates renal ischemia-reperfusion injury (IRI) in mouse models, but the effect of increasing CD55 and CD59 activity has not been examined. In this study, we investigated the impact of overexpression of human (h) CD55 ± hCD59 or treatment with soluble rhCD55 in a mouse model of renal IRI. Unilaterally nephrectomised mice were subjected to 18 (mild IRI) or 22 min (moderate IRI) warm renal ischemia, and analyzed 24 h after reperfusion for renal function (serum creatinine and urea), complement deposition (C3b/c and C9), and infiltration of neutrophils and macrophages. Transgenic mice expressing hCD55 alone were protected a..View full abstract
Awarded by Swiss National Science Foundation
This work was supported by grants from the Swiss National Science Foundation (Fellowship P2BEP3_155459) and the National Health and Medical Research Council of Australia.