Depletion of p21-activated kinase 1 up-regulates the immune system of APC(Delta 14/ ) mice and inhibits intestinal tumorigenesis
Nhi Huynh, Kai Wang, Mildred Yim, Chelsea J Dumesny, Mauro S Sandrin, Graham S Baldwin, Mehrdad Nikfarjam, Hong He
BMC CANCER | BIOMED CENTRAL LTD | Published : 2017
BACKGROUND: P21-activated kinase 1 (PAK1) stimulates growth and metastasis of colorectal cancer (CRC) through activation of multiple signalling pathways. Up-regulation of CRC stem cell markers by PAK1 also contributes to the resistance of CRC to 5-fluorouracil. The aim of this study was to investigate the effect of PAK1 depletion and inhibition on the immune system and on intestinal tumour formation in APC∆14/+ mice. METHODS: The PAK1 KO APC∆14/+ mice were generated by cross-breeding of PAK1 KO mice with APC∆14/+ mice. Splenic lymphocytes were analysed by flow cytometry, and immunohistochemical staining. The numbers of intestinal tumours were counted. Blood cells were also counted. RESULTS: ..View full abstract
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This work was supported by grants from the National Health and Medical Research Council of Australia (1,041,831, GSB/AS; 1,020,983, GSB), and the Austin Medical Research Foundation (HH).