Journal article

Transmembrane features governing Fc receptor CD16A assembly with CD16A signaling adaptor molecules

Alfonso Blazquez-Moreno, Soohyung Park, Wonpil Im, Melissa J Call, Matthew E Call, Hugh T Reyburn

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2017

Abstract

Many activating immunoreceptors associate with signaling adaptor molecules like FcεR1γ or CD247. FcεR1γ and CD247 share high sequence homology and form disulphide-linked homodimers that contain a pair of acidic aspartic acid residues in their transmembrane (TM) domains that mediate assembly, via interaction with an arginine residue at a similar register to these aspartic acids, with the activating immunoreceptors. However, this model cannot hold true for receptors like CD16A, whose TM domains do not contain basic residues. We have carried out an extensive site-directed mutagenesis analysis of the CD16A receptor complex and now report that the association of receptor with the signaling adapto..

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Grants

Awarded by Ministerio de Economia y Competitividad (MINECO)


Awarded by MINECO PhD Studentship


Awarded by Australian Research Council Future Fellowship


Awarded by Extreme Science and Engineering Discovery Environment Grant


Awarded by National Science Foundation


Awarded by National Institutes of Health


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Funding Acknowledgements

We thank Dr. Cesar Santiago for helpful advice on design of the mutagenesis strategy; Dr. Mar Vales Gomez for critical reading of the manuscript; Ruth Lopez-Caro, Dr. Mario Mellado, Pilar Lucas, Dr. Laura Martinez-Munoz, and Dr. Blanca Soler-Palacios for constructive advice and help; and Dr. Gloria Esteso for daily encouragement and support. This work was supported by Ministerio de Economia y Competitividad (MINECO) Grant SAF2014-58752-R (to H.T.R.), MINECO PhD Studentship SVP-2014-068263 (to A. B.-M.), Australian Research Council Future Fellowship FT120100145 (to M.J.C.), Extreme Science and Engineering Discovery Environment Grant MCB070009 (to W.I.), National Science Foundation Grant MCB-1727508 (to W.I.), and National Institutes of Health Grant R01-GM092950 (to W.I.).