Journal article

Watson-Crick Base Pair Radical Cation as a Model for Oxidative Damage in DNA

Linda Feketeova, Bun Chan, George N Khairaah, Vincent Steinmetz, Philippe Maitre, Leo Radom, Richard AJ O'Hair

JOURNAL OF PHYSICAL CHEMISTRY LETTERS | AMER CHEMICAL SOC | Published : 2017

Abstract

The deleterious cellular effects of ionizing radiation are well-known, but the mechanisms causing DNA damage are poorly understood. The accepted molecular events involve initial oxidation and deprotonation at guanine sites, triggering hydrogen atom abstraction reactions from the sugar moieties, causing DNA strand breaks. Probing the chemistry of the initially formed radical cation has been challenging. Here, we generate, spectroscopically characterize, and examine the reactivity of the Watson-Crick nucleobase pair radical cation in the gas phase. We observe rich chemistry, including proton transfer between the bases and propagation of the radical site in deoxyguanosine from the base to the s..

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Grants

Awarded by French FT-ICR network (CNRS)


Funding Acknowledgements

We thank the Australian Research Council (ARC) for financial support through the ARC Centre of Excellence and Discovery programs. L.F. thanks the ARC for the award of an APD. G.N.K. thanks UPSud for a Visiting Scholar Fellowship. Financial support from the French FT-ICR network (FR3624 CNRS) is gratefully acknowledged. The authors gratefully acknowledge the generous allocation of computing time from the National Computational Infrastructure (NCI) National Facility, Intersect Australia Ltd, RIKEN ACCC, the Institute for Molecular Science, and the Victorian Partnership for Advanced Computing (VPAC).