Journal article

IMMATURE SURFACE IG B-CELLS CAN CONTINUE TO REARRANGE KAPPA-L-CHAIN AND LAMBDA-L-CHAIN GENE LOCI

A ROLINK, U GRAWUNDER, D HAASNER, A STRASSER, F MELCHERS

JOURNAL OF EXPERIMENTAL MEDICINE | ROCKEFELLER UNIV PRESS | Published : 1993

Abstract

Pro and pre B cells possess the long-term capacity to proliferate in vitro on stromal cells and interleukin 7 (IL-7) and can differentiate to surface immunoglobulin (sIg+) cells upon removal of IL-7 from the cultures. A key event in this differentiation is the extensive cell loss due to apoptosis. Because the proto-oncogene bcl-2 can promote cell survival, we established pre-B cell lines from E mu-bcl-2 transgenic mice. These pre-B cells have the same properties as those derived from non-bcl-2 transgenic mice except that they do not die by apoptosis. This allowed us to study the fate of newly formed B cells in vitro for a longer period of time. Here we show that early during the differentiat..

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University of Melbourne Researchers