Journal article

Effect of a bcl-2 transgene on production and localization of precursor B cells in mouse bone marrow

R Janani, AW Harris, A Strasser, S Dhanoa, R Plyam, DG Osmond

EXPERIMENTAL HEMATOLOGY | CARDEN JENNINGS PUBL CO LTD | Published : 1998

Abstract

Many B cell precursors die while differentiating in mouse bone marrow. To ascertain the mechanisms involved in this process, populations of B lineage cells and their tissue localization were analyzed in bone marrow of transgenic mice overexpressing the apoptosis inhibitor, Bcl-2. Immunofluorescence labeling and mitotic arrest were used to quantitate the number and proliferative activity of mu- pro-B cells (terminal deoxynucleotidyl transferase [TdT]+B220-, TdT+B220+, and TdT-B220+); pre-B cells (cmu+); and B cells (smu+). Mature B cells (IgM+IgD+) were increased 16- to 20-fold. In addition, immature B lymphocytes (IgM+IgD-/low), representing newly formed cells, were increased three- to sixfo..

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University of Melbourne Researchers