PB76: a novel surface glycoprotein preferentially expressed on mouse pre‐B cells and plasma cells detected by the monoclonal antibody G‐52

Abstract

A monoclonal antibody (mAb) G‐5‐2 was isolated which binds to transformed as well as normal cells of the B lineage but not to cells of the T cell, myeloid lineages nor to fibroblasts. mAb G‐5‐2 reacts with pre‐B and plasma cell‐transformed lines, and it preferentially recognizes normal pre‐B cells from fetal liver and bone marrow as well as plasma cells from spleen of mice. G‐5‐2+ fetal liver cells isolated by cell sorter express mRNA for μ heavy chain Ig gene and generate in vitro antibody‐producing cells when co‐cultured with lipopolysaccharide and rat thymocyte filler cells. During development the frequency and staining intensity of G‐5‐2+ cells in fetal liver from normal mice increases f..