Journal article

PB76 - A NOVEL SURFACE GLYCOPROTEIN PREFERENTIALLY EXPRESSED ON MOUSE PRE-B CELLS AND PLASMA-CELLS DETECTED BY THE MONOCLONAL-ANTIBODY G-5-2

A STRASSER

EUROPEAN JOURNAL OF IMMUNOLOGY | VCH PUBLISHERS INC | Published : 1988

Abstract

A monoclonal antibody (mAb) G-5-2 was isolated which binds to transformed as well as normal cells of the B lineage but not to cells of the T cell, myeloid lineages nor to fibroblasts. mAb G-5-2 reacts with pre-B and plasma cell-transformed lines, and it preferentially recognizes normal pre-B cells from fetal liver and bone marrow as well as plasma cells from spleen of mice. G-5-2+ fetal liver cells isolated by cell sorter express mRNA for mu heavy chain Ig gene and generate in vitro antibody-producing cells when co-cultured with lipopolysaccharide and rat thymocyte filler cells. During development the frequency and staining intensity of G-5-2+ cells in fetal liver from normal mice increases ..

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University of Melbourne Researchers