Journal article

Decreased c-Src expression enhances osteoblast differentiation and bone formation

M Marzia, NA Sims, S Voit, S Migliaccio, A Taranta, S Bernardini, T Faraggiana, T Yoneda, GR Mundy, BF Boyce, R Baron, A Teti

JOURNAL OF CELL BIOLOGY | ROCKEFELLER UNIV PRESS | Published : 2000

Abstract

c-src deletion in mice leads to osteopetrosis as a result of reduced bone resorption due to an alteration of the osteoclast. We report that deletion/reduction of Src expression enhances osteoblast differentiation and bone formation, contributing to the increase in bone mass. Bone histomorphometry showed that bone formation was increased in Src null compared with wild-type mice. In vitro, alkaline phosphatase (ALP) activity and nodule mineralization were increased in primary calvarial cells and in SV40-immortalized osteoblasts from Src(-/-) relative to Src(+/+) mice. Src-antisense oligodeoxynucleotides (AS-src) reduced Src levels by approximately 60% and caused a similar increase in ALP activ..

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