WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells
Abdulah J Alshawaf, Ana Antonic, Efstratios Skafidas, Dominic Chi-Hung Ng, Mirella Dottori
STEM CELLS INTERNATIONAL | HINDAWI LTD | Published : 2017
Mutations in WD40-repeat protein 62 (WDR62) are commonly associated with primary microcephaly and other developmental cortical malformations. We used human pluripotent stem cells (hPSC) to examine WDR62 function during human neural differentiation and model early stages of human corticogenesis. Neurospheres lacking WDR62 expression showed decreased expression of intermediate progenitor marker, TBR2, and also glial marker, S100β. In contrast, inhibition of c-Jun N-terminal kinase (JNK) signalling during hPSC neural differentiation induced upregulation of WDR62 with a corresponding increase in neural and glial progenitor markers, PAX6 and EAAT1, respectively. These findings may signify a role ..View full abstract
This study was supported by the University of Melbourne, Australian Research Council Future Fellowship, and Saudi Arabian Ministry of Higher Education (Saudi Arabia).