Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA
Erin E Verity, Sarina Camuglia, Catherine T Agius, Chi Ong, Robert Shaw, Ian Barr, Deborah Middleton, Steven Rockman
INFLUENZA AND OTHER RESPIRATORY VIRUSES | WILEY-BLACKWELL | Published : 2012
BACKGROUND: Vaccination is considered the most effective means of reducing influenza burden. The emergence of H5N1 and pandemic spread of novel H1N1/2009 viruses reinforces the need to have strategies in place to rapidly develop seed viruses for vaccine manufacture. METHODS: Candidate pandemic vaccine strains consisting of the circulating strain haemagglutinin (HA) and neuraminidase (NA) in an A/PR/8/34 backbone were generated using alternative synthetic DNA approaches, including site-directed mutagenesis of DNA encoding related virus strains, and rapid generation of virus using synthetic DNA cloned into plasmid vectors. RESULTS: Firstly, synthetic A/Bar Headed Goose/Qinghai/1A/2005 (H5N1) v..View full abstract
The authors would like to acknowledge S. Lowther, D. Johnson and R. Robinson (CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Victoria, Australia) for technical support. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health and Ageing.