Development and comparison of molecular assays for the rapid detection of the pandemic influenza A (H1N1) 2009 virus.
Wai-Yip Lam, Ting-Fan Leung, Nelson Lee, Jo LK Cheung, Apple CM Yeung, Yolanda II Ho, Rickjason CW Chan, Kitty SC Fung, Ian G Barr, David SC Hui, Joseph JY Sung, Paul KS Chan
J Med Virol | Published : 2010
Human infection with the novel pandemic influenza A (H1N1) virus was first identified in April 2009. Two months later, the World Health Organization (WHO) had raised the pandemic level to phase 6. Rapid case identification is essential for prompt patient management and public health actions. This study developed real-time and conventional reverse transcription-polymerase chain reaction (rRT-PCR and cRT-PCR) assays for pandemic H1N1 detection, and compared their sensitivities with protocols developed by WHO reference centres. Altogether, three rRT-PCR and one cRT-PCR targeting the matrix gene for universal detection of influenza A; three rRT-PCR, one cRT-PCR targeting the hemagglutinin (HA) g..View full abstract