Journal article
A commercial ELISA detects high levels of human H5 antibody but cross-reacts with influenza A antibodies
S Stelzer-Braid, B Wong, P Robertson, GW Lynch, K Laurie, R Shaw, I Barr, PW Selleck, C Baleriola, R Escott, G Katsoulotos, WD Rawlinson
Journal of Clinical Virology | ELSEVIER SCIENCE BV | Published : 2008
Abstract
Background and objectives: Commercial serological assays to determine influenza A H5N1 infection are available, although the accuracy and reproducibility of these are not reported in detail. This study aimed to assess the validity of a commercial ELISA H5 hemagglutinin (HA) antibody kit. Study design: A commercial ELISA for detection of antibodies towards influenza A H5 HA was evaluated using human sera from vaccinated individuals. The ELISA was used to screen 304 sera with elevated influenza A complement fixation titres collected between the period 1995-2007. Results and conclusions: The ELISA was found to be accurate for sera with high levels of anti-H5 antibodies, and would be useful in c..
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Funding Acknowledgements
The authors would like to thank the NH & MRC for support. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health and Ageing.