MDCK-SIAT1 cells show improved isolation rates for recent human influenza viruses compared to conventional MDCK cells.
Ding Yuan Oh, Ian G Barr, Jenny A Mosse, Karen L Laurie
Journal of Clinical Microbiology | Published : 2008
The ability to isolate and propagate influenza virus is an essential tool for the yearly surveillance of circulating virus strains and to ensure accurate clinical diagnosis for appropriate treatment. The suitability of MDCK-SIAT1 cells, engineered to express increased levels of alpha-2,6-linked sialic acid receptors, as an alternative to conventional MDCK cells for isolation of circulating influenza virus was assessed. A greater number of influenza A (H1N1 and H3N2) and B viruses from stored human clinical specimens collected between 2005 and 2007 were isolated following inoculation in MDCK-SIAT1 cells than in MDCK cells. In addition, a higher titer of virus was recovered following culture i..View full abstract