Journal article

Clinical failures associated with rpoB mutations in phenotypically occult multidrug-resistant Mycobacterium tuberculosis

DA Williamson, SA Roberts, JE Bower, R Vaughan, S Newton, O Lowe, CA Lewis, JT Freeman

The International Journal of Tuberculosis and Lung Disease | INT UNION AGAINST TUBERCULOSIS LUNG DISEASE (I U A T L D) | Published : 2012

Abstract

SETTING: Recently, Mycobacterium tuberculosis isolates have been described that test phenotypically susceptible to rifampicin (RMP) yet harbour genotypic rpoB mutations. OBJECTIVE: 1) To investigate the impact of such mutations on clinical outcomes among RMP-susceptible isolates, and 2) to determine the prevalence of rpoB mutations among isoniazid (INH) monoresistant isolates at our laboratory and to describe the association between the presence of these mutations and clinical outcomes. METHODS: M. tuberculosis isolates were screened for mutations in the rpoB gene using the Cepheid Gene-Xpert® MTB/RIF assay. Clinical correlation was made by reviewing patient case notes. RESULTS: Isolates fro..

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