Physiological Performance of a Detergent Decellularized Heart Valve Implanted for 15 Months in Vietnamese Pigs: Surgical Procedure, Follow-up, and Explant Inspection
Michele Gallo, Filippo Naso, Helen Poser, Antonio Rossi, Paolo Franci, Roberto Bianco, Matteo Micciolo, Fabio Zanella, Umberto Cucchini, Luca Aresu, Edward Buratto, Roberto Busetto, Michele Spina, Alessandro Gandaglia, Gino Gerosa
Artificial Organs | WILEY-BLACKWELL | Published : 2012
This study features the longest experimental follow-up for decellularized heart valves implanted in an animal model. Porcine aortic heart valves were decellularized according to a disclosed standardized method in which TRITON X-100 and sodium cholate (TRICOL) are used in succession, followed by a further treatment with the endonuclease Benzonase to completely remove the nucleic acid remnants. Experimental animals (n = 17), represented by Vietnamese pigs (VPs), received a decellularized aortic allograft as a substitute for the replacement of their right ventricular outflow tract. The surgical implantation of the TRICOL-treated aortic valve conduit was successful in 11 VPs, while perioperative..View full abstract
Awarded by Regione Veneto
The authors wish to thank Dr. Ludovico Scenna for the legislative support in the management of animal welfare. We are also thankful to Dr. Roberto Barbieri, Dr. Alessandro Carlotto, and Dr. Franco Salvatore (Veterinarian ULSS 15, Alta Padovana), Dr. Massimo Marchesan, Mazzon Bros.' stud farm (Piazzola s/B, Padova, Italy), Zanato's stud farm (Ponso, Padova, Italy) and Guerriero Bros.' slaughterhouse (Villafranca Padovana, Padova, Italy) for the generous supply of swine hearts. This research was supported by the Italian grants Azione Biotech III and RSF 286/08 from the Regione Veneto. All authors do not have any conflicts of interests to disclose.