Journal article

A small set of extra-embryonic genes defines a new landmark for bovine embryo staging

SA Degrelle, KA LêCao, Y Heyman, RE Everts, E Campion, C Richard, C Ducroix-Crépy, XC Tian, HA Lewin, JP Renard, C Robert-Granié, I Hue

Reproduction | BIOSCIENTIFICA LTD | Published : 2011

Abstract

Axis specification in mouse is determined by a sequence of reciprocal interactions between embryonic and extra-embryonic tissues so that a few extra-embryonic genes appear as 'patterning' the embryo. Considering these interactions as essential, but lacking in most mammals the genetically driven approaches used in mouse and the corresponding patterning mutants, we examined whether a molecular signature originating from extra-embryonic tissues could relate to the developmental stage of the embryo proper and predict it. To this end, we have profiled bovine extra-embryonic tissues at peri-implantation stages, when gastrulation and early neurulation occur, and analysed the subsequent expression p..

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University of Melbourne Researchers

Grants

Awarded by INRA


Awarded by French Ministry of Research


Awarded by USDA CSREES National Research Initiative


Awarded by USDA ARS


Funding Acknowledgements

This work was supported by awards from INRA (no. 20 058 to JP Renard), the French Ministry of Research (no. 20085 to I Hue and J-P Renard), the EU (SABRE to J-P Renard), USDA CSREES National Research Initiative Grant 2002-35205-11548 and USDA ARS contracts AG 58-1265-2-018 and 58-1265-2-020. The support of the International Relationship Direction of INRA is warmly acknowledged. S A Degrelle was an MNERT fellow. K-A Le Cao was a PhD student with a shared CNRS/INRA (PHASE-GA) fellowship.