A pilot study on transcriptome data analysis of folliculogenesis in pigs
G Tosser-Klopp, K-A Le Cao, A Bonnet, N Gobert, F Hatey, C Robert-Granie, S Dejean, J Antic, L Baschet, M SanCristobal
ANIMAL | CAMBRIDGE UNIV PRESS | Published : 2009
Three different stages of pig antral follicles have been studied in a granulosa-cell transcriptome analysis on nylon microarrays (1152 clones). The data have been generated from seven RNA follicle pools and several technical replicates were made. The objective of this paper was to state the feasibility of a transcriptomic protocol for the study of folliculogenesis in the pig. A statistical analysis was chosen, relying on the linear mixed model (LMM) paradigm. Low variability within technical replicates was hence checked with a LMM. Relevant genes that might be involved in the studied process were then selected. For the most significant genes, statistical methods such as principal component a..View full abstract
We thank the Genopole de Toulouse-Midi Pyrenees for technical support, and especially Cecile Donnadieu-Tonon. We thank the SIGENAE team (www.sigenae.org) for the help in annotation and submitting data to the GEO database. We thank Helene Quesnel and Herve Demay for providing ovaries. We are grateful to Herve Lefebvre, Claire Rogel-Gaillard and Helene Berges for their help in generating microarrays. We also thank an anonymous referee for his helpful suggestions. This work was in part funded by the ACI IMP BIO.