Uncoupled Embryonic and Extra-Embryonic Tissues Compromise Blastocyst Development after Somatic Cell Nuclear Transfer
Severine A Degrelle, Florence Jaffrezic, Evelyne Campion, Kim-Anh Le Cao, Daniel Le Bourhis, Christophe Richard, Nathalie Rodde, Renaud Fleurot, Robin E Everts, Jerome Lecardonnel, Yvan Heyman, Xavier Vignon, Xiangzhong Yang, Xiuchun C Tian, Harris A Lewin, Jean-Paul Renard, Isabelle Hue
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2012
Somatic cell nuclear transfer (SCNT) is the most efficient cell reprogramming technique available, especially when working with bovine species. Although SCNT blastocysts performed equally well or better than controls in the weeks following embryo transfer at Day 7, elongation and gastrulation defects were observed prior to implantation. To understand the developmental implications of embryonic/extra-embryonic interactions, the morphological and molecular features of elongating and gastrulating tissues were analysed. At Day 18, 30 SCNT conceptuses were compared to 20 controls (AI and IVP: 10 conceptuses each); one-half of the SCNT conceptuses appeared normal while the other half showed signs ..View full abstract
Awarded by French Ministry of Research
Awarded by USDA CSREES National Research Initiative
Awarded by USDA ARS
This work was supported by awards from the French Ministry of Research (no. 20085 to IH and J-PR), the EU (SABRE to J-PR), the USDA CSREES National Research Initiative Grant 2002-35205-11548 and USDA ARS contracts (AG 58-1265-2-018 to XY and XCT and 58-1265-2-020 to HAL). SAD was a MNERT fellow, KALC was a PhD student with a shared CNRS/INRA (PHASE-GA) fellowship, and NR was a technical assistant supported by the EMBIC European network of excellence headed at the INRA by O. Sandra. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.