Journal article

Increased methylation and decreased expression of homeobox genes TLX1, HOXA10 and DLX5 in human placenta are associated with trophoblast differentiation

Boris Novakovic, Thierry Fournier, Lynda K Harris, Joanna James, Claire T Roberts, Hannah EJ Yong, Bill Kalionis, Daniele Evain-Brion, Peter R Ebeling, Euan M Wallace, Richard Saffery, Padma Murthi

SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2017

Abstract

Homeobox genes regulate embryonic and placental development, and are widely expressed in the human placenta, but their regulatory control by DNA methylation is unclear. DNA methylation analysis was performed on human placentae from first, second and third trimesters to determine methylation patterns of homeobox gene promoters across gestation. Most homeobox genes were hypo-methylated throughout gestation, suggesting that DNA methylation is not the primary mechanism involved in regulating HOX genes expression in the placenta. Nevertheless, several genes showed variable methylation patterns across gestation, with a general trend towards an increase in methylation over gestation. Three genes (T..

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Grants

Awarded by National Health and Medical Research Council (NHMRC, Australia)


Awarded by NHMRC Senior Research Fellowship


Awarded by CTR by a NHMRC Senior Research Fellowship


Awarded by NHMRC


Awarded by Biotechnology and Biological Sciences Research Council


Funding Acknowledgements

B.N. was supported by a National Health and Medical Research Council (NHMRC, Australia) C. J. Martin Early Career Fellowship (GNT1072966), R.S. by a NHMRC Senior Research Fellowship (GNT1045161) and CTR by a NHMRC Senior Research Fellowship (GNT1020749). The Murdoch Childrens Research Institute is supported by the Victorian Government's Operational Infrastructure Support Program. P.M. was supported by NHMRC Project Grant #509140.