Use of a high-throughput screen to identify Leptospira mutants unable to colonize the carrier host or cause disease in the acute model of infection
Renee A Marcsisin, Thanatchaporn Bartpho, Dieter M Bulach, Amporn Srikram, Rasana W Sermswan, Ben Adler, Gerald L Murray
JOURNAL OF MEDICAL MICROBIOLOGY | MICROBIOLOGY SOC | Published : 2013
The molecular basis for leptospirosis infection and colonization remains poorly understood, with no efficient methods available for screening libraries of mutants for attenuation. We analysed the attenuation of leptospiral transposon mutants in vivo using a high-throughput method by infecting animals with pooled sets of transposon mutants. A total of 95 mutants was analysed by this method in the hamster model of acute infection, and one mutant was identified as attenuated (M1233, lb058 mutant). All virulence factors identified in Leptospira to date have been characterized in the acute model of infection, neglecting the carrier host. To address this, a BALB/c mouse colonization model was esta..View full abstract
Carla Osinski and Timothy Witchell are thanked for technical assistance. This work was supported by the National Health and Medical Research Council and the Australian Research Council, Canberra, Australia.