Journal article
Phosphoproteomic profiling of mouse primary HSPCs reveals new regulators of HSPC mobilization
Leo D Wang, Scott B Ficarro, John N Hutchinson, Roland Csepanyi-Komi, Phi T Nguyen, Eva Wisniewski, Jessica Sullivan, Oliver Hofmann, Erzsebet Ligeti, Jarrod A Marto, Amy J Wagers
BLOOD | AMER SOC HEMATOLOGY | Published : 2016
Abstract
Protein phosphorylation is a central mechanism of signal transduction that both positively and negatively regulates protein function. Large-scale studies of the dynamic phosphorylation states of cell signaling systems have been applied extensively in cell lines and whole tissues to reveal critical regulatory networks, and candidate-based evaluations of phosphorylation in rare cell populations have also been informative. However, application of comprehensive profiling technologies to adult stem cell and progenitor populations has been challenging, due in large part to the scarcity of such cells in adult tissues. Here, we combine multicolor flow cytometry with highly efficient 3-dimensional hi..
View full abstractGrants
Awarded by Damon Runyon-Sohn Foundation Cancer Research Fellowship
Awarded by Alex's Lemonade Stand Foundation Young Investigator Award
Awarded by St. Baldrick's Foundation Scholar Award
Awarded by Hungarian Research Fund
Awarded by NIH (National Heart, Lung, and Blood Institute)
Awarded by National Institute of Diabetes and Digestive and Kidney Diseases
Awarded by National Cancer Institute
Awarded by NIH National Human Genome Research Institute
Awarded by CSD Consortium
Funding Acknowledgements
This study was supported by a Damon Runyon-Sohn Foundation Cancer Research Fellowship (DSRG 02-12), an Alex's Lemonade Stand Foundation Young Investigator Award (31582), a St. Baldrick's Foundation Scholar Award (243625) (L.D.W.), and grants from the Hungarian Research Fund (OTKA K108382) (E.L.), the NIH (National Heart, Lung, and Blood Institute R01HL088582 and National Institute of Diabetes and Digestive and Kidney Diseases P30DK036836 [A.J.W.], and National Cancer Institute R21CA188881 and R21CA178860 [J.A.M.]), and the Dana-Farber Strategic Research Initiative (J.A.M.). Grants from the NIH National Human Genome Research Institute to Velocigene at Regeneron Inc. (U01HG004085) and the CSD Consortium (U01HG004080) funded the generation of targeted embryonic stem cells for 8500 genes in the KOMP program archived/distributed by the KOMP Repository (UC Davis and Children's Hospital Oakland Research Institute, U42RR024244, National Center for Research Resources). J.N.H. was partially supported by funding from the Harvard Stem Cell Institute through the Center for Stem Cell Bioinformatics.