Clonal dynamics of native haematopoiesis
Jianlong Sun, Azucena Ramos, Brad Chapman, Jonathan B Johnnidis, Linda Le, Yu-Jui Ho, Allon Klein, Oliver Hofmann, Fernando D Camargo
NATURE | NATURE PUBLISHING GROUP | Published : 2014
It is currently thought that life-long blood cell production is driven by the action of a small number of multipotent haematopoietic stem cells. Evidence supporting this view has been largely acquired through the use of functional assays involving transplantation. However, whether these mechanisms also govern native non-transplant haematopoiesis is entirely unclear. Here we have established a novel experimental model in mice where cells can be uniquely and genetically labelled in situ to address this question. Using this approach, we have performed longitudinal analyses of clonal dynamics in adult mice that reveal unprecedented features of native haematopoiesis. In contrast to what occurs fo..View full abstract
Awarded by NIH
Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
Awarded by OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
We are grateful to members of the Camargo laboratory, L. Zon, S. Orkin, M. Goodell and F. Mercier for comments. We thank R. Mathew for cell sorting and Y. Fujiwara for transgenic injections (supported by NIH P30 DK049216). We thank Z. Izsvak (Max-Delbruck-Center) for HSB expression vector and M. Kay (Stanford University) for transposon plasmid. This work was supported by the NIH Director's New Innovator Award (DP2OD006472) to F.D.C. and funds from the Harvard Stem Cell Institute to B.C. and O.H.