Cell-specific translational profiling in acute kidney injury
Jing Liu, A Michaela Krautzberger, Shannan H Sui, Oliver M Hofmann, Ying Chen, Manfred Baetscher, Ivica Grgic, Sanjeev Kumar, Benjamin Humphreys, Winston A Hide, Andrew R McMahon
JOURNAL OF CLINICAL INVESTIGATION | AMER SOC CLINICAL INVESTIGATION INC | Published : 2014
Acute kidney injury (AKI) promotes an abrupt loss of kidney function that results in substantial morbidity and mortality. Considerable effort has gone toward identification of diagnostic biomarkers and analysis of AKI-associated molecular events; however, most studies have adopted organ-wide approaches and have not elucidated the interplay among different cell types involved in AKI pathophysiology. To better characterize AKI-associated molecular and cellular events, we developed a mouse line that enables the identification of translational profiles in specific cell types. This strategy relies on CRE recombinase-dependent activation of an EGFP-tagged L10a ribosomal protein subunit, which allo..View full abstract
Awarded by California Institute of Regenerative Medicine
Awarded by Deutsche Forschungsgemeinschaft
This research was supported by funds from the Harvard Stem Cell Institute and the California Institute of Regenerative Medicine (53-5178-7980) to A.P. McMahon. I. Grgic was supported by a fellowship from the Deutsche Forschungsgemeinschaft (GR 3301/4-1). The authors wish to thank members of the Harvard Genome Modification Facility for their technical assistance; Fan Gao, Yibu Chen, and Meng Li at University of Southern California for their bioinformatics assistance; and the Washington University George M. O'Brien Center for Kidney Disease Research for creatinine assays.