Journal article

Chronic Endotoxin Exposure Produces Airflow Obstruction and Lung Dendritic Cell Expansion

Peggy S Lai, Jennifer M Fresco, Miguel A Pinilla, Alvaro A Macias, Ronald D Brown, Joshua A Englert, Oliver Hofmann, James A Lederer, Winston Hide, David C Christiani, Manuela Cernadas, Rebecca M Baron

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY | AMER THORACIC SOC | Published : 2012

Abstract

Little is known about the mechanisms of persistent airflow obstruction that result from chronic occupational endotoxin exposure. We sought to analyze the inflammatory response underlying persistent airflow obstruction as a result of chronic occupational endotoxin exposure. We developed a murine model of daily inhaled endotoxin for periods of 5 days to 8 weeks. We analyzed physiologic lung dysfunction, lung histology, bronchoalveolar lavage fluid and total lung homogenate inflammatory cell and cytokine profiles, and pulmonary gene expression profiles. We observed an increase in airway hyperresponsiveness as a result of chronic endotoxin exposure. After 8 weeks, the mice exhibited an increase ..

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University of Melbourne Researchers

Grants

Awarded by National Institutes of Health (National Institute of Allergy and Infectious Diseases) grant


Awarded by National Institutes of Health (National Heart, Lung, and Blood Institute) grant


Awarded by Centers for Disease Control (National Institute for Occupational Safety and Health) grants


Awarded by National Institutes of Health (National Institute of Environmental Health Sciences) grant


Awarded by NATIONAL HEART, LUNG, AND BLOOD INSTITUTE


Awarded by NATIONAL INSTITUTE FOR OCCUPATIONAL SAFETY AND HEALTH


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Awarded by NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES


Funding Acknowledgements

This work was supported by National Institutes of Health (National Institute of Allergy and Infectious Diseases) grant K08 AI054465 (R.M.B.), National Institutes of Health (National Heart, Lung, and Blood Institute) grant R01 HL091957 (R.M.B.), Centers for Disease Control (National Institute for Occupational Safety and Health) grants R01 OH 002421 (D.C.C.) and T42OH008416 (D.C.C.), and National Institutes of Health (National Institute of Environmental Health Sciences) grant P42 00002 (D.C.C.).