Complexes of gastrin with In3 , Ru3 or Ga3 ions are not recognised by the cholecystokinin 2 receptor
Marie Laval, Kathryn M Marshall, John Sachinidis, Andrew Scott, Mal Eutick, Graham S Baldwin
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY | SPRINGER | Published : 2017
The peptide hormone gastrin (Gamide) binds trivalent metal ions, including indium (In), ruthenium (Ru) and gallium (Ga), with high affinity. Complexes of gastrin with chelated isotopes of In and Ga have previously been used for the location of tumours expressing the cholecystokinin 2 receptor (CCK2R). The aim of the present study was to purify the complexes of Gamide with radioactive isotopes of In, Ru or Ga and to investigate their ability to bind to the CCK2R. The radioactive Gamide complexes were purified on Sep-Pak C18 cartridges or by anion exchange HPLC. Binding to the CCK2R was assessed with a stably transfected clone of the gastric carcinoma cell line AGS. The 106Ru-Gamide complex co..View full abstract
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Awarded by National Health and Medical Research Council of Australia
This work was supported by the National Health and Medical Research Council of Australia [Grant Number 1020983 to GSB].