Journal article

Assessment of oxidative stress biomarkers in exhaled breath condensate and blood of dairy heifer calves from birth to weaning

Rujuta Ranade, Saranika Talukder, Gary Muscatello, Pietro Celi

The Veterinary Journal | ELSEVIER SCI LTD | Published : 2014

Abstract

The balance between oxidants and antioxidants may be impaired in neonatal calves but only limited information is available on physiological changes in oxidative biomarkers in healthy calves. The aim of this study was to measure biomarkers of oxidative stress (OS) in calves from birth to weaning. Eighteen, healthy, female Holstein-Friesian calves were used in the study. Blood and exhaled breath condensate (EBC) samples were taken on the day of birth (Week 0) and then at Weeks 6, 12 and 18. Reactive oxygen metabolites (ROMs) and biological antioxidant potential (BAP) were determined on fresh blood and the degree of OS was expressed as an oxidative stress index (OSI), where OSI = ROMs/BAP × 100..

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Grants

Funding Acknowledgements

The authors wish to acknowledge the Honda Foundation for providing financial support to purchase the Free Radical Elective Evaluator System and the Australian Companion Animal Health Foundation and the Faculty of Veterinary Science, University of Sydney for providing financial support to purchase the ECoScreen and EcoVent. We are also grateful to Feng Li for his assistance with the OXY-ads and SHp assays.