Interactions between nutritional and opioidergic pathways in the control of LH secretion in male sheep
Pietro Celi, David W Miller, Dominique Blache, Graeme B Martin
Animal Reproduction Science | ELSEVIER SCIENCE BV | Published : 2010
Our aim was to determine the role of opioidergic processes in the effects of nutrition on the secretion of LH pulses in the mature male sheep. In the first of three experiments, adult Merino rams were acclimatised to a maintenance diet and then allocated to one of three dietary groups (n=5): continuation of the maintenance diet (Group M); reduction to half of the maintenance allocation (Group HM); or supplementation of the maintenance diet with lupin grain (Group HD). An initial administration of naloxone (2mg/kg body weight, i.v.) was followed at 40-min intervals by three further administrations (1mg/kg). Blood was sampled every 20 min for 12h before the initial naloxone administration and ..View full abstract
The animal experiments described here would not have been possible without help willingly provided by everyone in the Animal Science Group, especially Margaret Blackberry. This work was supported by the National Health & Medical Research Council of Australia. Pietro Celi was supported by an International Postgraduate Research Scholarship and David Miller by a University Postgraduate Award, both from UWA.